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FORENSIC SCIENCE DISCIPLINES
141
sources.25 From this base of experience, the fingerprint community asserts
that the latent print examiner learns to judge whether there is sufficient
detail (which varies with image quality) to make a source determination
during the evaluation phase of ACE-V.
The latent print community in the United States has eschewed numeri-
cal scores and corresponding thresholds, because those developed to date26
have been based only on minutia, not on the unique features of the fric-
tion ridge skin (e.g., lengths of ridges, shapes of ridges, crease lengths and
shapes, scar lengths and shapes). Additionally, thresholds based on count-
ing the number of features that correspond, lauded by some as being more
“objective,” are still based on primarily subjective criteria—an examiner
must have the visual expertise to discern the features (most important in
low-clarity prints) and must determine that they are indeed in agreement.
A simple point count is insufficient for characterizing the detail present in
a latent print; more nuanced criteria are needed, and, in fact, likely can be
determined.
Reporting of Results
SWGFAST has promulgated three acceptable conclusions resulting from
latent print comparison: individualization (or identification), exclusion, or
inconclusive.27 Although adherence to this standard is common, some latent
print examiners report either “identification” or “negative” results. “Nega-
tive” (or sometimes “not identified”) is an ambiguous conclusion, and it
could mean excluded, inconclusive, or unable to locate after exhaustive
search. It is problematic that the meaning of “negative” may be specific to
a particular agency, examiner, or case.
Latent print examiners report an individualization when they are con-
fident that two different sources could not have produced impressions with
the same degree of agreement among details. This is a subjective assessment.
There has been discussion regarding the use of statistics to assign match
probabilities based on population distributions of certain friction ridge
features. Current published statistical models, however, have not matured
past counts of corresponding minutia and have not taken clarity into con-
sideration. (This area is ripe for additional research.) As a result, the friction
ridge community actively discourages its members from testifying in terms
of the probability of a match; when a latent print examiner testifies that two
25 T. Busey and J. Vanderkolk. 2005. Behavioral and electrophysiological evidence for con-
figural processing in fingerprint experts. Vision Research 45:431-448.
26 See, e.g., I.W. Evett and R.A. Williams. 1996. A review of the sixteen points fingerprint
standard in England and Wales. Journal of Forensic Identification 46(1):49-73.
SWGFAST. 2002. Friction Ridge Examination Methodology for Latent Print Examiners.
142
STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
impressions “match,” they are communicating the notion that the prints
could not possibly have come from two different individuals.
As noted in Chapter 3, Jennifer Mnookin of the University of Califor-
nia, Los Angeles School of Law summarized the reporting of fingerprint
analyses as follows:
At present, fingerprint examiners typically testify in the language of ab-
solute certainty. Both the conceptual foundations and the professional
norms of latent fingerprinting prohibit experts from testifying to identifica-
tion unless they believe themselves certain that they have made a correct
match. Experts therefore make only what they term ‘positive’ or ‘absolute’
identifications—essentially making the claim that they have matched the
latent print to the one and only person in the entire world whose fingertip
could have produced it . . . Given the general lack of validity testing for
fingerprinting; the relative dearth of difficult proficiency tests; the lack of
a statistically valid model of fingerprinting; and the lack of validated stan-
dards for declaring a match, such claims of absolute, certain confidence in
identification are unjustified . . . Therefore, in order to pass scrutiny under
Daubert, fingerprint identification experts should exhibit a greater degree
of epistemological humility. Claims of ‘absolute’ and ‘positive’ identifica-
tion should be replaced by more modest claims about the meaning and
significance of a ‘match.’28
Summary Assessment
Historically, friction ridge analysis has served as a valuable tool, both
to identify the guilty and to exclude the innocent. Because of the amount of
detail available in friction ridges, it seems plausible that a careful compari-
son of two impressions can accurately discern whether or not they had a
common source. Although there is limited information about the accuracy
and reliability of friction ridge analyses, claims that these analyses have zero
error rates are not scientifically plausible.
ACE-V provides a broadly stated framework for conducting friction
ridge analyses. However, this framework is not specific enough to qualify as
a validated method for this type of analysis. ACE-V does not guard against
bias; is too broad to ensure repeatability and transparency; and does not
guarantee that two analysts following it will obtain the same results. For
these reasons, merely following the steps of ACE-V does not imply that one
is proceeding in a scientific manner or producing reliable results. A recent
28 J.L. Mnookin. 2008. The validity of latent fingerprint identification: Confessions of
a fingerprinting moderate. Law, Probability and Risk 7:127. See also the discussion in C.
Champod. 2008. Fingerprint examination: Towards more transparency. Law Probability and
Risk 7:111-118.
FORENSIC SCIENCE DISCIPLINES
143
paper by Haber and Haber29 presents a thorough analysis of the ACE-V
method and its scientific validity. Their conclusion is unambiguous: “We
have reviewed available scientific evidence of the validity of the ACE-V
method and found none.”30 Further, they state:
[W]e report a range of existing evidence that suggests that examiners differ
at each stage of the method in the conclusions they reach. To the extent
that they differ, some conclusions are invalid. We have analysed the ACE-
V method itself, as it is described in the literature. We found that these
descriptions differ, no single protocol has been officially accepted by the
profession and the standards upon which the method’s conclusions rest
have not been specified quantitatively. As a consequence, at this time the
validity of the ACE-V method cannot be tested.31
Recent legal challenges, New Hampshire vs. Richard Langill32 and
Maryland vs. Bryan Rose,33 have also highlighted two important issues for
the latent print community: documentation and error rate. Better documen-
tation is needed of each step in the ACE-V process or its equivalent. At the
very least, sufficient documentation is needed to reconstruct the analysis,
if necessary. By documenting the relevant information gathered during the
analysis, evaluation, and comparison of latent prints and the basis for the
conclusion (identification, exclusion, or inconclusive), the examiner will
create a transparent record of the method and thereby provide the courts
with additional information on which to assess the reliability of the method
for a specific case. Currently, there is no requirement for examiners to
document which features within a latent print support their reasoning and
conclusions.
Error rate is a much more difficult challenge. Errors can occur with
any judgment-based method, especially when the factors that lead to the
ultimate judgment are not documented. Some in the latent print community
argue that the method itself, if followed correctly (i.e., by well-trained ex-
aminers properly using the method), has a zero error rate. Clearly, this as-
sertion is unrealistic, and, moreover, it does not lead to a process of method
improvement. The method, and the performance of those who use it, are
inextricably linked, and both involve multiple sources of error (e.g., errors
in executing the process steps, as well as errors in human judgment).
Some scientific evidence supports the presumption that friction ridge
patterns are unique to each person and persist unchanged throughout a
29 Mnookin, op. cit.
30 Ibid., p. 19.
31 Ibid.
32 157 N.H. 77, 945 A.2d 1 (N.H., April 04, 2008).
33 No. K06-0545 (MD Cir. Ct. Oct. 19, 2007).
144
STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
lifetime.34 Uniqueness and persistence are necessary conditions for friction
ridge identification to be feasible, but those conditions do not imply that
anyone can reliably discern whether or not two friction ridge impressions
were made by the same person. Uniqueness does not guarantee that prints
from two different people are always sufficiently different that they cannot
be confused, or that two impressions made by the same finger will also be
sufficiently similar to be discerned as coming from the same source. The
impression left by a given finger will differ every time, because of inevi-
table variations in pressure, which change the degree of contact between
each part of the ridge structure and the impression medium. None of these
variabilities—of features across a population of fingers or of repeated im-
pressions left by the same finger—has been characterized, quantified, or
compared.35
To properly underpin the process of friction ridge identification, ad-
ditional research is also needed into ridge flow and crease pattern distribu-
tions on the hands and feet. This information could be used to limit the
possible donor population of a particular print in a statistical approach
and could provide examiners with a more robust understanding of the
prevalence of different ridge flows and crease patterns. Additionally, more
research is needed regarding the discriminating value of the various ridge
formations and clusters of ridge formations.36 This would provide examin-
ers with a solid basis for the intuitive knowledge they have gained through
experience and provide an excellent training tool. It also would lead to a
good framework for future statistical models and provide the courts with
additional information to consider when evaluating the reliability of the
science. Recently, research has begun to build some of this basis.37
34 F. Galton. 1892. Fingerprints. New York: MacMillan; H. Cummins and C. Midlo. 1943.
Finger Prints, Palms and Soles: An Introduction of Dermatoglyphics. Philadelphia: The Blakis-
ton Company; A. Hale. 1952. Morphogenesis of volar skin in the human fetus. The American
Journal of Anatomy 91:147-173; S. Holt and L.S. Penrose. 1968. The Genetics of Dermal
Ridges. Springfield, IL: Charles C Thomas Publishing; W. Montagna and P. Parakkal. 1974.
The Structure and Function of Skin. New York: Academic Press; J. Raser and E. O’Shea. 2005.
Noise in gene expression: Origins, consequences, and control. Science 39:2010-2013.
35 Some in the friction ridge community point to an unpublished 1999 study by the Lock-
heed-Martin Corporation, the “50K vs. 50K Fingerprint Comparison Test,” as evidence of
the scientific validity of fingerprint “matchup.” But that study has several major design and
analysis flaws, as pointed out in D.H. Kaye. 2003. Questioning a courtroom proof of the
uniqueness of fingerprints. International Statistical Review 71(3):524. Moreover, even if it
were valid, the study provides only a highly optimistic estimate of the reliability of friction
ridge analyses, biased toward highly favorable conditions.
36 Haber and Haber also provide a sensible research agenda for enhancing the validity of
fingerprint comparisons.
37 E.g., C. Neumann, C. Champod, R. Puch-Solis, N. Egli, A. Anthonioz, and A. Bromage-
Griffiths. 2007. Computation of likelihood ratios in fingerprint identification for configu-
rations of any number of minutiae. Journal of Forensic Sciences 52(1):54-64; N.M. Egli,
FORENSIC SCIENCE DISCIPLINES
145
There is also considerable room for research on the various factors that
affect the quality of latent prints (e.g., condition of the skin, residue, me-
chanics of touch). Formal research could provide examiners with additional
tools to support or refute distortion explanations. Currently, distortion and
quality issues are typically based on “common sense” explanations or on
information that is passed down through oral tradition from examiner to
examiner. A criticism of the latent print community is that the examiners
can too easily explain a “difference” as an “acceptable distortion” in order
to make an identification.38
OTHER PATTERN/IMPRESSION EVIDENCE:
SHOEPRINTS AND TIRE TRACKS
Other pattern evidence, also referred to as impression evidence, occurs
when an object such as a shoe or a tire leaves an impression at the crime
scene or on another object or a person. Impressions can be either two
dimensional, such as shoeprints in dust, or three dimensional, such as tire
track impressions in mud. Shoeprints and tire tracks are common types of
impression evidence examined by forensic examiners, but the list of po-
tential types of impression evidence is long. Examples include bite marks,
markings on bullets and cartridge cases, ear prints, lip prints, toolmarks,
some bloodstain patterns, and glove prints.39 Although there are general
approaches concerning the analytical sequence of various types of impres-
sion evidence, each has its own set of characteristics. For example, some
types of impression evidence, such as those arising from footwear and tires,
require knowledge of manufacturing and wear, while other types, such as
ear prints and bloodstain patterns, do not. Because footwear and tire track
impressions comprise the bulk of the examinations conducted, the remarks
in this section are specifically focused on these analyses. Bite marks, mark-
ings on bullets and cartridge cases, and bloodstain patterns are covered in
later sections in this chapter.
C. Champod, and P. Margot. 2007. Evidence evaluation in fingerprint comparison and auto-
mated fingerprint identification systems—Modelling within finger variability. Forensic Science
International 167(2-3):189-195.
38 U.S. Department of Justice, Office of the Inspector General. 2006. A Review of the FBI’s
Handling of the Brandon Mayfield Case. Office of the Inspector General Oversight and Review
Division, January.
39 M. Liukkonen, H. Majamaa, and J. Virtanen. 1996. The role and duties of the shoeprint/
toolmark examiner in forensic laboratories. Forensic Science International 82:99-108.
146
STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
Sample Data and Collection
Impression evidence at the scene is generally of two types: latent (invis-
ible to the naked eye) or patent (visible). The quality of impression evidence
left at the scene cannot be controlled, but failures in the initial scene work
used to collect, preserve, and possibly enhance the evidence will degrade the
quality of the evidence eventually used for comparative analysis. After doc-
umentation at the scene, the evidence is preserved and possibly enhanced
using techniques such as those based on chemistry (e.g., metal detection),
physical characteristics (e.g., super glue fuming, powder dusting, casting),
or transfer onto a contrasting surface (e.g., electrostatic transfer or gel lift-
ing). The quality of the enhanced impression that is used for comparison
will depend largely on the experience, training, and scientific knowledge of
the scene investigator as well as the agency’s resources.
Although some analysis of impression evidence might begin at the
scene, the comparison of scene evidence to known exemplars occurs in the
laboratory. The educational background of forensic scientists who examine
shoeprints and tire track impressions runs the gamut from a high school
diploma to scientists with Ph.D.s. Identifications are largely subjective and
are based on the examiner’s experience and on the number of individual,
identifying characteristics in common with a known standard.
Analyses
The goal of impression evidence analysis is to identify a specific source
of the impression, and the analytical process that this follows generally is
an accepted sequence: identifying the class (group) characteristics of the
evidence, followed by locating and comparing individual, identifying (also
termed accidental or random) characteristics.40
Class characteristics of footwear and tires result from repetitive, con-
trolled processes that are typically mechanical, such as those used to manu-
facture items in quantity. Although defined similarly by various authors,
Bodziak describes footwear class characteristics as “an intentional or un-
avoidable characteristic that repeats during the manufacturing process and
is shared by one or more other shoes.”41 For tires, Nause defines class char-
acteristics as, “[p]hysical characteristics acquired during the manufacturing
process (made from the same mold) that tires have in common.”42 He con-
tinues, “Class characteristics can often be combined to limit a tire impres-
sion to a very select group within the overall group bearing similar class
40 Ibid.
41 W.J. Bodziak. 1999. Footwear Impression Evidence-Detection, Recovery, and Examina-
tion. Boca Raton, FL: CRC Press, 2nd ed., p. 329.
42 Nause, op. cit.
FORENSIC SCIENCE DISCIPLINES
147
characteristics. (In the field of forensic tire evidence, class characteristics
often refer to such things as design, pattern, size, shape, mold variations,
etc.).”43 Regardless of the type of impression evidence, class characteristics
are not sufficient to conclude that any one particular shoe or tire made
the impression. That latter step—which is not always possible—requires
comparison of the individual identifying characteristics on the impression
evidence with those on a shoe or tire that is suspected of leaving the im-
pression. These individual characteristics occur during the normal use of
an item, sometimes called wear and tear,44 and are created by “random,
uncontrolled processes.”45 For footwear, Bodziak writes that “individual
identifying characteristics are characteristics that result when something is
randomly added to or taken away from a shoe outsole that either causes
or contributes to making that shoe outsole unique.”46 Such characteristics
might include cuts, scratches, gouges, holes, or random inclusions that
result from manufacturing, such as bubbles, and those that result from
adherent substances, such as rocks, chewing gum, papers, or twigs.
Following analysis of the impression, an identification is determined or
ruled out according to the number of individual characteristics the evidence
has in common with the suspected source. But there is no defined threshold
that must be surpassed, nor are there any studies that associate the number
of matching characteristics with the probability that the impressions were
made by a common source. It is generally accepted that the specific number
of characteristics needed to assign a definite positive identification depends
on the quality and quantity of these accidental characteristics and the cri-
teria established by individual laboratories.47 According to Cassidy, many
factors and accidental characteristics are required before a positive identi-
fication can be established; however, the most important are the examiner’s
experience, the clarity of the impression, and the uniqueness of the charac-
teristic.48 Proficiency testing for examiners of impression evidence is avail-
able through Collaborative Testing Service, Inc., but the proficiency tests
for footwear impressions include samples that are either a match or not a
match49—that is, none of the samples included in the tests have the sort of
ambiguities that would lead an experienced examiner to an “inconclusive”
43 Ibid.
44 M.J. Cassidy. 1980. Footwear Identification. Quebec, Canada: Government Printing
Office Centre.
45 K. Inman and N. Rudin. 2001. Principles and Practice of Criminalistics. Boca Raton, FL:
CRC Press, p. 129.
46 Ibid., p. 335.
47 Liukkonen, Majamaa, and Virtanen, op. cit.
48 Cassidy, op. cit.
49 H. Majamaa and Y. Anja. 1996. Survey of the conclusions drawn of similar footwear
cases in various crime laboratories. Forensic Science International 82:109-120.
148
STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
conclusion. IAI has a certification program for footwear and tire track ex-
aminers.50 The group’s recommended course of study has 13 segments, and
each segment includes a suggested reading list and practical and/or written
exercises. The student must pass an examination. This course of study does
not require an understanding of the scientific basis of the examinations,
and it does not recommend the use of a scientific method. Also, there is no
provision or recommendation for proficiency testing or continuing educa-
tion. SWGTREAD, a group of footwear and tire track examiners formed by
the FBI, recommends that a trainee candidate have (1) a bachelor’s degree
(preferably in a physical or natural science) from an accredited college or
university; or (2) an associate degree or 60 college semester hours, plus two
years of job-related forensic experience; or (3) a high school diploma or
equivalent, plus four years of job-related forensic experience.51
Scientific Interpretation and Reporting of Results
For footwear evidence, Fawcett52 and Bodziak53 have attempted to
assign probabilistic or statistical significance to impression comparisons.
Generally, shoeprint and tire track examiners prefer nonstatistical language
to report or to testify to the result of their findings. Terms such as “positive
identification” and “nonidentification” can be used to indicate an identifica-
tion or nonidentification, respectively, and “nonconclusive” would indicate
situations in which the analysis falls short of either of the other two.54
In a European survey, examiners were given identical mock cases. Ac-
cidental, identifying characteristics were purposely put onto the sole of
new shoes, and examiners were asked to make a statement concerning the
strength of matches. The results of the survey concluded that there were
considerable differences in the conclusions reached by different laboratories
examining identical cases.”55 SWGTREAD recommends terminology such
as:
“identification” (definite conclusion of identity)
“probably made” (very high degree of association)
50 Recommended Course of Study for Footwear & Tire Track Examiners. 1995. Mendota
Heights, MN: International Association for Identification.
51 SWGTread. 2006. Guide for Minimum Qualifications and Training for a Forensic
Footwear and/or Tire Tread Examiner. Available at www.theiai.org/guidelines/swgtread/
qualifications_final.pdf.
52 A.S. Fawcett. 1970. The role of the footmark examiner. Journal of the Forensic Science
Society 10:227-244.
53 Bodziak, op. cit., pp. 342-346.
54 Ibid.
55 H. Majamaa and Y. Anja., op. cit.
FORENSIC SCIENCE DISCIPLINES
149
“could have made” (significant association of multiple class
characteristics)
“inconclusive” (limited association of some characteristics)
“probably did not make” (very high degree of nonassociation)
“elimination” (definite exclusion)
”unsuitable” (lacks sufficient detail for a meaningful comparison).
Additionally, SWGTREAD discourages the use of once common ter-
minology, such as “consistent with” (acceptable when used to describe
a similarity of characteristics), “match/no match,” “responsible for/not
responsible for,” and “caused with/not caused with.”56 Neither the IAI nor
SWGTREAD address the statistical evaluation of impression evidence.
Summary Assessment
The scientific basis for the evaluation of impression evidence is that
mass-produced items (e.g., shoes, tires) pick up features of wear that,
over time, individualize them. However, because these features continue
to change as they are worn, elapsed time after a crime can undercut the
forensic scientist’s certainty. At the least, class characteristics can be identi-
fied, and with sufficiently distinctive patterns of wear, one might hope for
specific individualization. However, there is no consensus regarding the
number of individual characteristics needed to make a positive identifica-
tion, and the committee is not aware of any data about the variability of
class or individual characteristics or about the validity or reliability of the
method. Without such population studies, it is impossible to assess the
number of characteristics that must match in order to have any particular
degree of confidence about the source of the impression.
Experts in impression evidence will argue that they accumulate a sense
of those probabilities through experience, which may be true. However,
it is difficult to avoid biases in experience-based judgments, especially in
the absence of a feedback mechanism to correct an erroneous judgment.
These problems are exacerbated with the less common types of impression
evidence. For example, a European survey found that 42 laboratories con-
ducted 28,093 shoeprint examinations and 41 laboratories conducted 591
tire track examinations, but only 14 laboratories conducted a total of 21 lip
print examinations and 17 laboratories conducted a total of 100 ear print
examinations.57 Although one might argue that those who perform the
56 SWGTREAD. 2006. Standard Terminology for Expressing Conclusions of Forensic
Footwear and Tire Impression Examinations. Available at www.theiai.org/guidelines/swgtread/
terminology_final.pdf.
57 Liukkonen, Majamaa, and Virtanen, op. cit.
150
STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
work in laboratories that conduct hundreds or thousands of evaluations of
impression evidence develop useful experience and judgment, it is difficult
to assert that the field has enough collective judgment about the variabilities
in lip prints and ear prints based on tens of examinations. The community
simply does not have enough data about the natural variability of those less
frequent impressions, absent the presence of a clear deformity or scar, to
infer whether the observed degree of similarity is significant.
Most of the research in the field is conducted in forensic laboratories,
with the results published in trade journals, such as the Journal of Forensic
Identification. With regard to reporting, SWGTREAD is moving toward the
use of standard language to convey the conclusions reached.58 But neither
IAI nor SWGTREAD addresses the issue of what critical research should be
done or by whom, critical questions that should be addressed include the
persistence of individual characteristics, the rarity of certain characteristic
types, and the appropriate statistical standards to apply to the significance
of individual characteristics. Also, little if any research has been done to
address rare impression evidence. Much more research on these matters is
needed.
TOOLMARK AND FIREARMS IDENTIFICATION
Toolmarks are generated when a hard object (tool) comes into contact
with a relatively softer object. Such toolmarks may occur in the commis-
sion of a crime when an instrument such as a screwdriver, crowbar, or wire
cutter is used or when the internal parts of a firearm make contact with the
brass and lead that comprise ammunition. The marks left by an implement
such as a screwdriver or a firearm’s firing pin depend largely on the manu-
facturing processes—and manufacturing tools—used to create or shape it,
although other surface features (e.g., chips, gouges) might be introduced
through post-manufacturing wear. Manufacturing tools experience wear
and abrasion as they cut, scrape, and otherwise shape metal, giving rise
to the theory that any two manufactured products—even those produced
consecutively with the same manufacturing tools—will bear microscopically
different marks. Firearms and toolmark examiners believe that toolmarks
may be traced to the physical heterogeneities of an individual tool—that is,
that “individual characteristics” of toolmarks may be uniquely associated
with a specific tool or firearm and are reproduced by the use of that tool
and only that tool.
The manufacture and use of firearms produces an extensive set of
58 SWGTREAD. 2006. Standard Terminology for Expressing Conclusions of Forensic
Footwear and Tire Impression Examinations. Available at www.theiai.org/guidelines/swgtread/
terminology_final.pdf.
FORENSIC SCIENCE DISCIPLINES
151
specialized toolmarks. Gun barrels typically are rifled to improve accuracy,
meaning that spiral grooves are cut into the barrel’s interior. The process
of cutting these grooves into the barrel leaves marks and scrapes on the
relatively softer metal of the barrel.59 In turn, these markings are transferred
to the softer metal of a bullet as it exits the barrel. Over time, with repeated
use (and metal-to-metal scraping), the marks on a barrel (and the corre-
sponding “stria” imparted to bullets) may change as individual imperfec-
tions are formed or as cleanliness of the barrel changes. The brass exterior
of cartridge cases receive analogous toolmarks during the process of gun
firing: the firing pin dents the soft primer surface at the base of the cartridge
to commence firing, the primer area is forced backward by the buildup of
gas pressure (so that the texture of the gun’s breech face is impressed on
the cartridge), and extractors and ejectors leave marks as they expel used
cartridges and cycle in new ammunition.
Firearms examination is one of the more common functions of crime
laboratories. Even small laboratories with limited services often perform
firearms analysis. In addition to the analysis of marks on bullets and car-
tridges, firearms examination also includes the determination of the firing
distance, the operability of a weapon, and sometimes the analysis of primer
residue to determine whether someone recently handled a weapon. These
broader aspects are not covered here.
Sample and Data Collection
When a tool is used in a crime, the object that contains the tool marks
is recovered when possible. If a toolmark cannot be recovered, it can be
photographed and cast. Test marks made by recovered tools can be made
in a laboratory and compared with crime scene toolmarks.
In the early 1990s, the FBI and the Bureau of Alcohol, Tobacco, Fire-
arms, and Explosives (ATF) developed separate databases of images of
bullet and cartridge case markings, which could be queried to suggest pos-
sible matches. In 1996, the National Institute of Standards and Technology
(NIST) developed data exchange standards that permitted the integration
of the FBI’s DRUGFIRE database (cartridge case images) and the ATF’s
CEASEFIRE database (then limited to bullet images). The current National
Integrated Ballistic Information Network (NIBIN) includes images from
both cartridge cases and bullets that are associated with crime scenes and
is maintained by the ATF.
Periodically—and particularly in the wake of the Washington, DC,
59 Although the metal and initial rifling are very similar, the cutting of the individual barrels,
the finishing machining, and the cleaning and polishing begin the process of differentiation of
the two sequentially manufactured barrels.
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STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
sniper attacks in 2002—the question has been raised of expanding the scope
of databases like NIBIN to include images from test firings of newly manu-
factured firearms. In concept, this would permit downstream investigators
who recover a cartridge case or bullet at a crime scene to identify the likely
source firearm. Though two states (Maryland and New York) instituted
such reference ballistic image databases for newly manufactured firearms,
proposals to create such a database at the national level did not make sub-
stantial progress in Congress. A recent report of the National Academies,
Ballistic Imaging, examined this option in great detail and concluded that
“[a] national reference ballistic image database of all new and imported
guns is not advisable at this time.”60
Analyses
In both firearm and toolmark identification, it is useful to distinguish
several types of characteristics that are considered by examiners. “Class
characteristics” are distinctive features that are shared by many items of the
same type. For example, the width of the head of a screwdriver or the pat-
tern of serrations in the blade of a knife may be class characteristics that are
common to all screwdrivers or knives of a particular manufacturer and/or
model. Similarly, the number of grooves cut into the barrel of a firearm and
the direction of “twist” in those grooves are class characteristics that can
filter and restrict the range of firearms that match evidence found at a crime
scene. “Individual characteristics” are the fine microscopic markings and
textures that are said to be unique to an individual tool or firearm. Between
these two extremes are “subclass characteristics” that may be common to
a small group of firearms and that are produced by the manufacturing pro-
cess, such as when a worn or dull tool is used to cut barrel rifling.
Bullets and cartridge cases are first examined to determine which class
characteristics are present. If these differ from a comparison bullet or car-
tridge, further examination may be unnecessary. The microscopic markings
on bullets and cartridge cases and on toolmarks are then examined under a
comparison microscope (made from two compound microscopes joined by
a comparison bridge that allows viewing of two objects at the same time).
The unknown and known bullet or cartridge case or toolmark surfaces
are compared visually by a firearms examiner, who can evaluate whether
a match exists.
60 National Research Council. 2008. Ballistic Imaging. Washington, DC: The National
Academies Press, p. 5.
FORENSIC SCIENCE DISCIPLINES
153
Scientific Interpretation
The task of the firearms and toolmark examiner is to identify the indi-
vidual characteristics of microscopic toolmarks apart from class and sub-
class characteristics and then to assess the extent of agreement in individual
characteristics in the two sets of toolmarks to permit the identification of
an individual tool or firearm.
Guidance from the Association of Firearm and Tool Mark Examiners
(AFTE)61 indicates that an examiner may offer an opinion that a specific
tool or firearm was the source of a specific set of toolmarks or a particular
bullet striation pattern when “sufficient agreement” exists in the pattern
of two sets of marks. The standards then define agreement as significant
“when it exceeds the best agreement demonstrated between tool marks
known to have been produced by different tools and is consistent with the
agreement demonstrated by tool marks known to have been produced by
the same tool.”62
Knowing the extent of agreement in marks made by different tools, and
the extent of variation in marks made by the same tool, is a challenging
task. AFTE standards acknowledge that these decisions involve subjective
qualitative judgments by examiners and that the accuracy of examiners’
assessments is highly dependent on their skill and training. In earlier years,
toolmark examiners relied on their past casework to provide a foundation
for distinguishing between individual, class, and subclass characteristics.
More recently, extensive training programs using known samples have
expanded the knowledge base of examiners.
The emergence of ballistic imaging technology and databases such as
NIBIN assist examiners in finding possible candidate matches between
pieces of evidence, including crime scene exhibits held in other geographic
locations. However, it is important to note that the final determination of
a match is always done through direct physical comparison of the evidence
by a firearms examiner, not the computer analysis of images. The growth
of these databases also permits examiners to become more familiar with
similarities in striation patterns made by different firearms. Newer imag-
ing techniques assess toolmarks using three-dimensional surface measure-
ment data, taking into account the depth of the marks. But even with
more training and experience using newer techniques, the decision of the
toolmark examiner remains a subjective decision based on unarticulated
61 Theory of identification, range of striae comparison reports and modified glossary
definitions—An AFTE Criteria for Identification Committee report. 1992. Journal of the As-
sociation of Firearm and Tool Mark Examiners 24:336-340.
62 Ibid., p. 336.
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STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
standards and no statistical foundation for estimation of error rates.63
The National Academies report, Ballistic Imaging, while not claiming to
be a definitive study on firearms identification, observed that, “The valid-
ity of the fundamental assumptions of uniqueness and reproducibility of
firearms-related toolmarks has not yet been fully demonstrated.” That
study recognized the logic involved in trying to compare firearms-related
toolmarks by noting that, “Although they are subject to numerous sources
of variability, firearms-related toolmarks are not completely random and
volatile; one can find similar marks on bullets and cartridge cases from the
same gun,” but it cautioned that, “A significant amount of research would
be needed to scientifically determine the degree to which firearms-related
toolmarks are unique or even to quantitatively characterize the probability
of uniqueness.”64
Summary Assessment
Toolmark and firearms analysis suffers from the same limitations dis-
cussed above for impression evidence. Because not enough is known about
the variabilities among individual tools and guns, we are not able to specify
how many points of similarity are necessary for a given level of confidence
in the result. Sufficient studies have not been done to understand the reli-
ability and repeatability of the methods. The committee agrees that class
characteristics are helpful in narrowing the pool of tools that may have
left a distinctive mark. Individual patterns from manufacture or from wear
might, in some cases, be distinctive enough to suggest one particular source,
but additional studies should be performed to make the process of individu-
alization more precise and repeatable.
63 Recent research has attempted to develop a statistical foundation for assessing the likeli-
hood that more than one tool could have made specific marks by assessing consecutive match-
ing striae, but this approach is used in a minority of cases. See A.A. Biasotti. 1959. A statistical
study of the individual characteristics of fired bullets. Journal of Forensic Sciences 4:34; A.A.
Biasotti and J. Murdock. 1984. “Criteria for identification” or “state of the art” of firearms
and tool marks identification. Journal of the Association of Firearms and Tool Mark Exam-
iners 16(4):16; J. Miller and M.M. McLean. 1998. Criteria for identification of tool marks.
Journal of the Association of Firearms and Tool Mark Examiners 30(1):15; J.J. Masson. 1997.
Confidence level variations in firearms identification through computerized technology. Journal
of the Association of Firearms and Tool Mark Examiners 29(1):42. For a critique of this area
and a comparison of scientific issues involving toolmark evidence and DNA evidence, see A.
Schwartz. 2004-2005. A systemic challenge to the reliability and admissibility of firearms and
tool marks identification. Columbia Science and Technology Law Review 6:2. For a rebuttal
to this critique, see R.G. Nichols. 2007. Defending the scientific foundations of the firearms
and tool mark identification discipline: Responding to recent challenges. Journal of Forensic
Sciences 52(3):586-594.
64 All quotes from National Research Council. 2008. Ballistic Imaging. Washington, DC:
The National Academies Press, p. 3.
FORENSIC SCIENCE DISCIPLINES
155
A fundamental problem with toolmark and firearms analysis is the
lack of a precisely defined process. As noted above, AFTE has adopted a
theory of identification, but it does not provide a specific protocol. It says
that an examiner may offer an opinion that a specific tool or firearm was
the source of a specific set of toolmarks or a bullet striation pattern when
“sufficient agreement” exists in the pattern of two sets of marks. It defines
agreement as significant “when it exceeds the best agreement demonstrated
between tool marks known to have been produced by different tools and
is consistent with the agreement demonstrated by tool marks known to
have been produced by the same tool.” The meaning of “exceeds the best
agreement” and “consistent with” are not specified, and the examiner is
expected to draw on his or her own experience. This AFTE document,
which is the best guidance available for the field of toolmark identification,
does not even consider, let alone address, questions regarding variability,
reliability, repeatability, or the number of correlations needed to achieve a
given degree of confidence.
Although some studies have been performed on the degree of similarity
that can be found between marks made by different tools and the vari-
ability in marks made by an individual tool, the scientific knowledge base
for toolmark and firearms analysis is fairly limited. For example, a report
from Hamby, Brundage, and Thorpe65 includes capsule summaries of 68
toolmark and firearms studies. But the capsule summaries suggest a heavy
reliance on the subjective findings of examiners rather than on the rigorous
quantification and analysis of sources of variability. Overall, the process for
toolmark and firearms comparisons lacks the specificity of the protocols
for, say, 13 STR DNA analysis. This is not to say that toolmark analysis
needs to be as objective as DNA analysis in order to provide value. And,
as was the case for friction ridge analysis and in contrast to the case for
DNA analysis, the specific features to be examined and compared between
toolmarks cannot be stipulated a priori. But the protocols for DNA analysis
do represent a precisely specified, and scientifically justified, series of steps
that lead to results with well-characterized confidence limits, and that is the
goal for all the methods of forensic science.
ANALYSIS OF HAIR EVIDENCE
The basis for hair analyses as forensic evidence stems from the fact that
human and animal hairs routinely are shed and thus are capable of being
65 J.E. Hamby, D.J. Brundage, and J.W. Thorpe. 2009. The identification of bullets fired
from 10 consecutively rifled 9mm Ruger pistol barrels—A research project involving 468
participants from 19 countries. Available online at http://www.fti-ibis.com/DOWNLOADS/
Publications/10%20Barrel%20Article-%20a.pdf.
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transferred from an individual to the crime scene, and from the crime scene
to an individual. Forensic hair examiners generally recognize that various
physical characteristics of hairs can be identified and are sufficiently differ-
ent among individuals that they can be useful in including, or excluding,
certain persons from the pool of possible sources of the hair. The results of
analyses from hair comparisons typically are accepted as class associations;
that is, a conclusion of a “match” means only that the hair could have come
from any person whose hair exhibited—within some levels of measurement
uncertainties—the same microscopic characteristics, but it cannot uniquely
identify one person. However, this information might be sufficiently useful
to “narrow the pool” by excluding certain persons as sources of the hair.
Although animal hairs might provide useful evidence in certain cases
(e.g., animal poaching), animal hair analysis often can lead to an identifica-
tion of only the type of animal, not the specific breed66; consequently, most
(90 to 95 percent) of hair analyses refer to analyses of human hair. Human
hairs from different parts of the body have different characteristics; Houck
cautions strongly against drawing conclusions about hairs from one part of
the body based on analyses of hairs from a different body part.67
Houck and Bisbing recommend as minimal training for hair examiners
a bachelor’s degree in a natural or applied science (e.g., chemistry, biology,
forensic science), on-the-job training programs, and an annual proficiency
test.68
Sample Data and Collection
Sample hairs received for analysis initially are examined macroscopi-
cally for certain broad features such as color, shaft form (e.g., straight,
wavy, curved, kinked), length, and overall shaft thickness (e.g., fine, me-
dium, coarse).
In the second stage of analysis, hairs are mounted on microscopic slides
using a mounting medium that has the same refractive index (about 1.54)
as the hair, to better view the microscopic features (see next section). One
hair or multiple hairs from the same source may be mounted on a glass
microscope slide with an appropriate cover slip, as long as each mounted
hair is clearly visible. It is most important that questioned and known hairs
are mounted in the same type of mounting medium.
During this examination, the hair analyst attempts to identify the part
of the body from which the hair might have come, based on certain de-
66 P.D. Barnett and R.R. Ogle. 1982. Probabilities and human hair comparison. Journal of
Forensic Sciences 27(2):272-278.
67 M.M. Houck and R.E. Bisbing. 2005. Forensic human hair examination and comparison
in the 21st century. Forensic Science Review 17(1):7.
68 Ibid., p. 12.
FORENSIC SCIENCE DISCIPLINES
157
finable characteristics that distinguish hairs from various body locations.
Occasionally, suspects can be eliminated on the basis of these simple mi-
croscopic characteristics.
A “control” or “comparison” group of hairs must be collected from a
known hair source. A known head hair sample should consist of hairs from
the five different areas of the scalp (top, front, back including nape, and
both sides). Known hair samples should be obtained by a combination of
pulling and combing from the sampled region. Ideally, a total of 50 hairs
should be obtained from the scalp. A known pubic hair sample or a sample
from any other somatic region should ideally consist of 25 hairs obtained
by pulling and combing from different regions. A comparison can still be
performed with less than the recommended number of hairs, but this may
increase the likelihood of a false exclusion.69
Features from human hair analyses can be divided broadly into “ma-
jor characteristics” and “secondary characteristics.” The former category
includes features such as color, treatment (e.g., dyed, bleached, curled,
permed), pigment aggregation (e.g., streaked, clumped, patchy), and shaft
form (e.g., wavy, straight, curly). Other major characteristics may include
pigment distribution (e.g., uniform, peripheral, clustered), medulla appear-
ance, if present (e.g., continuous, interrupted, or fragmented—and opaque
or translucent), hair diameter, medullary index, and presence or absence of
cortical fusi (e.g., root or shaft). Secondary characteristics include cuticular
margin (e.g., smooth, serrated, looped, or cracked), pigment density (e.g.,
absent, sparse, heavy), pigment size (e.g., absent, fine, coarse), tip shape
(e.g., tapered, cut, rounded, frayed, split), and shaft diameter (e.g., narrow
or wide).70
Studies of Accuracy in Identification
In 1974, investigators Gaudette and Keeping described a system of hair
analysis and used it in a study of pairwise comparisons among 861 hairs
from 100 different persons.71 They acknowledged that “the hair samples
were not chosen from the population at random, but were selected so that
the probability of two hairs being similar would be greater, if anything,
than in the population at large.”72 From their assignment of probabilities,
the authors estimated that the chance of asserting a difference between two
69 Scientific Working Group on Materials Analysis (SWGMAT). 2005. Forensic human
hair examination guidelines. Forensic Science Communications 7(2). Available at www.fbi.
gov/hq/lab/fsc/backissu/april2005/standards/2005_04_standards02.htm.
70 Ibid.
71 B.D. Gaudette and E.S. Keeping. 1974. An attempt at determining probabilities in human
scalp hair comparison. Journal of Forensic Sciences 19(3):599-606.
72 Ibid., p. 65.
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STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
hairs from the same person is small, about 1 in 4,500.73 This assignment of
probabilities has since been shown to be unreliable.74 Moreover, the study
does not confirm the chance of asserting a match between two dissimilar
hairs, and the authors acknowledge that, “due to the fact that so many of
the characteristics coded are subjective—for example, color, texture—it was
not possible to get complete reproducibility between two or more examin-
ers coding the same hair.”75
Barnett and Ogle raised four concerns with the Gaudette and Keeping
study: (1) it relied on idealized (not from real life) test scenarios; (2) there
was no objective basis for selecting the features; (3) the statistical analysis
of data from the study was questionable; and (4) there was a possible exam-
iner bias.76 Gaudette attempted to address these concerns through a further
study. However, this additional study involved only three hair examiners,
in addition to the author. The author concluded that:
. . . whereas hair is not generally a basis for positive personal identification, the
presence of abnormalities or unusual features or the presence of a large number of
different unknown hairs all similar to the standard can lead to a more positive con-
clusion. The problem, at present, lies in finding suitable additional characteristics
[of hair, for effecting individualization]. Although there is basic agreement as to the
value of the macroscopic and microscopic characteristics used, other characteristics
are either unreliable or controversial. Physical characteristics such as refractive
index, density, scale counts, tensile strength, and electrical properties have been
proposed by some workers but have been attacked by others, and the general con-
sensus is that they are of little use in hair comparison.77
In 1990, Wickenheiser and Hepworth attempted a study to address
examiner bias in a small study with only two examiners. They reported that
“no incorrect associations were made by either examiner.”78 But a study
with only two examiners cannot offer accurate and precise estimates of bias
in the population of examiners.
An attempt at an objective system for identifying “matches” among
hair samples is presented in Verma et al., based on a neural network.79
73 A later study on human pubic hairs (Caucasian only) estimated this probability as “about
1 in 800.” B.D. Gaudette. 1976. Probabilities and human pubic hair comparisons. Journal of
Forensic Sciences 21(3):514-517.
74 P.D. Barnett and R.R. Ogle. 1982. Probabilities and human hair comparison. Journal of
Forensic Sciences 27(2):272-278.
75 Gaudette and Keeping, op. cit.
76 Barnett and Ogle, op. cit.
77 B.D. Gaudette. 1978. Some further thoughts on probabilities and human hair compari-
sons. Journal of Forensic Sciences 23(4):758-763, pp. 761-762.
78 Wickenheiser and Hepworth, op. cit., p. 1327.
79 M.S. Verma, L. Pratt, C. Ganesh, and C. Medina. 2002. Hair-MAP: A prototype au-
tomated system for forensic hair comparison and analysis. Forensic Science International
129:168-186.
FORENSIC SCIENCE DISCIPLINES
159
According to the authors of this article, “The system accurately judged
whether two populations of hairs came from the same person or from dif-
ferent persons 83 percent of the time.”80 The article states that 83 percent
was obtained by testing the neural network on all possible pairs among 9
samples of hairs from 9 people (i.e., 81 combinations, of which 9 are “true
matches” and 72 are “true mismatches”). Their Table 381 can be summa-
rized as follows:
System said
System said
“same”
“different”
Same person
5
4
Total= 9
Different persons
9
64
Total=73
Because the total of these 4 numbers is 82, not 81, one presumes a
typographical error in the table; as stated, the number of correct calls is
(5 + 64)/81=0.85, or 85 percent. (If one of the counts, 5 or 64, is off by 1,
the percentage would be 84 percent.) However, the table also shows that
the neural network claimed 9 of the 73 different pairs as “same,” for a false
positive rate of 9/73=12 percent, and 4 sets of hairs from the same person
as “different,” for a false negative rate of 4/9=44 percent. With such high
error rates, one would want to study improvements to such systems before
putting them into routine practice.
Houck et al. indicate that proficiency testing is conducted regularly
for hair experts in crime laboratories.82 Collaborative Testing Services83
offers hair and fiber proficiency tests annually. Unfortunately, mass produc-
tion of test samples such as hair is problematic. Because known samples
exhibit a range of characteristics within each of the major and secondary
characteristics, it is not possible to provide comparable samples to multiple
examiners.
Scientific Interpretation and Reporting of Results
The success of hair analyses to make a positive identification is lim-
ited in important ways. Most hair examiners would opine only that hairs
exhibiting the same microscopic characteristics “could” have come from a
80 Ibid., p. 179.
81 Ibid., p. 180.
82 M.M. Houck, R.E. Bisbing, T.G. Watkins, and R.P. Harman. 2004. Locard exchange: The
science of forensic hair comparisons and the admissibility of hair comparison evidence: Frye
and Daubert considered. Modern Microscopy Journal Available at www.modernmicroscopy.
com/main.asp?article=36&searchkeys=Houck%2BBisbing.
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particular individual. Moreover, the “best” or most reliable characteristics
will vary by case. For example, “color” may be a critical determinant in a
case where it is artificial, because that introduces additional independent
variables, such as the time since treatment and the actual hair color, while
a natural hair might provide less information.
However, several members of the committee have experienced court-
room cases in which, despite the lack of a statistical foundation, microscopic
hair examiners have made probabilistic claims based on their experience,
as occurred in some DNA exoneration cases in which microscopic hair
analysis evidence had been introduced during trial. Aitken and Robertson
discuss some probabilistic concepts with respect to hair analysis.84
The availability of DNA analysis has lessened the reliance on hair
examination. In a very high proportion of cases involving hair evidence,
DNA can be extracted, even years after the crime has been committed.
Although the DNA extraction may consist of only mitochondrial DNA
(mtDNA), such analyses are likely to be much more specific than those
conducted on the physical features of hair. For this reason, cases that might
have relied heavily on hair examinations have been subjected more recently
to additional analyses using DNA.85 Because of the inherent limitations of
hair comparisons and the availability of higher-quality and higher-accuracy
analyses based on mtDNA, traditional hair examinations may be presented
less often as evidence in the future, although microscopic comparison of
physical features will continue to be useful for determining which hairs are
sufficiently similar to merit comparisons with DNA analysis and for exclud-
ing suspects and assisting in criminal investigations.
Summary Assessment
No scientifically accepted statistics exist about the frequency with
which particular characteristics of hair are distributed in the population.
There appear to be no uniform standards on the number of features on
which hairs must agree before an examiner may declare a “match.” In one
study of validity and accuracy of the technique, the authors required exact
agreement on seven “major” characteristics and at least two agreements
among six “secondary” characteristics.86 The categorization of hair features
depends heavily on examiner proficiency and practical experience.
An FBI study found that, of 80 hair comparisons that were “associ-
84 C.G.G. Aitken and J.A. Robertson. 1986. A contribution to the discussion of probabilities
and human hair comparisons. Journal of Forensic Sciences 32(3):684-689.
85 M.M. Houck and B. Budowle. 2002. Correlation of microscopic and mitochondrial DNA
hair comparisons. Journal of Forensic Sciences 47(5):964-967.
86 R.A. Wickenheiser and D.G. Hepworth. 1990. Further evaluation of probabilities in hu-
man hair comparisons. Journal of Forensic Sciences 35(6):1323-1329.
FORENSIC SCIENCE DISCIPLINES
161
ated” through microscopic examinations, 9 of them (12.5 percent) were
found in fact to come from different sources when reexamined through
mtDNA analysis.87 This illustrates not only the imprecision of microscopic
hair analyses, but also the problem with using imprecise reporting terminol-
ogy such as “associated with,” which is not clearly defined and which can
be misunderstood to imply individualization.
In some recent cases, courts have explicitly stated that microscopic hair
analysis is a technique generally accepted in the scientific community.88 But
courts also have recognized that testimony linking microscopic hair analysis
with particular defendants is highly unreliable.89 In cases where there seems
to be a morphological match (based on microscopic examination), it must
be confirmed using mtDNA analysis; microscopic studies alone are of lim-
ited probative value. The committee found no scientific support for the use
of hair comparisons for individualization in the absence of nuclear DNA.
Microscopy and mtDNA analysis can be used in tandem and may add to
one another’s value for classifying a common source, but no studies have
been performed specifically to quantify the reliability of their joint use.
ANALYSIS OF FIBER EVIDENCE
Fibers associated with a crime—including synthetic fibers such as nylon,
polyester and acrylic as well as botanical fibers such as ramie or jute, which
are common in ropes or twines—can be examined microscopically in the
same way as hairs, and with the same limitations. However, fibers also can
be analyzed using the tools of analytical chemistry, which provide a more
solid scientific footing than that underlying morphological examination. In
some cases, clothing and carpets have been subjected to relatively distinctive
environmental conditions (e.g., sunlight exposure or laundering agents) that
impart characteristics that can distinguish particular items from others from
the same manufacturing lot. Fiber examiners agree, however, that none of
these characteristics is suitable for individualizing fibers (associating a fiber
from a crime scene with one, and only one, source) and that fiber evidence
can be used only to associate a given fiber with a class of fibers.90
87 Houck and Budowle, op. cit.
88 E.g., State v. West, 877 A.2d 787 (Conn. 2005); Bookins v. State, 922 A.2d 389 (Del.
Supr, 2007).
89 See P.C. Giannelli and E. West. 2001. Hair comparison evidence. Criminal Law Bulletin
37:514.
90 See, e.g., R.R. Bresee. 1987. Evaluation of textile fiber evidence: A review. Journal of
Forensic Sciences 32(2):510-521. See also SWGMAT. 1999. Introduction to forensic fiber
examination. Forensic Science Communications 1(1). Available at www.fbi.gov/hq/lab/fsc/
backissu/april1999/houcktoc.htm, which includes the following summarization in Section 5.4:
“It can never be stated with certainty that a fiber originated from a particular textile because
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STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
Another type of fiber analysis consists of physically matching two rem-
nants that appear to be torn from one another. By comparing the shapes of
the mating edges, and aligning any patterns in the cloth, it can sometimes be
possible to associate a fragment with the garment or other item from which
it was torn. This is a form of pattern matching, analogous to the matching
of shoe and tire prints, but it will not be discussed further here.
Sample Collection and Analysis
The collection of fibers and of a comparison group follows the same
procedures as those for mounting hairs. If a macroscopic analysis (e.g., or
color, texture, shape) suggests that the two samples appear to be the same,
additional procedures such as the following are pursued:
1. Microscopy (reflected light)
2. Polarized light microscopy/fluorescence microscopy
3. Infrared microscopy (to determine man-made fiber composition,
such as nylon, polyester)
4. Solubility in a medium
5. Melting point
6. Cross-sectional shape
7. Pyrolysis GC
8. Microspectrophotometry (MSP)
9. Raman spectroscopy
The last of these, Raman spectroscopy, often can provide additional
information on polymer chain length (short, medium, long) and branching.
Its use in forensic laboratories is rare, although research is under way to
develop possible applications. A good overview of fiber evidence is provided
by Grieve and Robertson.91
Summary Assessment
A group of experienced paint examiners, the Fiber Subgroup of the
Scientific Working Group on Materials Analysis (SWGMAT), has produced
guidelines,92 but no set standards, for the number and quality of character-
other textiles are produced using the same fiber types and color. The inability to positively
associate a fiber to a particular textile to the exclusion of all others, however, does not mean
that a fiber association is without value.”
91 M. Grieve and J. Robertson. 1999. Forensic Examination of Fibres. London: Taylor and
Francis Ltd.
92 SWGMAT, op. cit. Available at www.fbi.gov/hq/lab/fsc/backissu/april1999/houcktoc.
htm.
FORENSIC SCIENCE DISCIPLINES
163
istics that must correspond in order to conclude that two fibers came from
the same manufacturing batch. There have been no studies of fibers (e.g.,
the variability of their characteristics during and after manufacturing) on
which to base such a threshold. Similarly, there have been no studies to
inform judgments about whether environmentally related changes discerned
in particular fibers are distinctive enough to reliably individualize their
source, and there have been no studies that characterize either reliability or
error rates in the procedures. Thus, a “match” means only that the fibers
could have come from the same type of garment, carpet, or furniture; it can
provide only class evidence.
Because the analysis of fibers is made largely through well-character-
ized methods of chemistry, it would be possible in principle to develop an
understanding of the uncertainties associated with those analyses.93 How-
ever, to date, that has not been done. Fiber analyses are reproducible across
laboratories because there are standardized procedures for such analyses.
Proficiency tests are routinely provided and taken annually, and the reports
are available from Collaborative Testing Services.
QUESTIONED DOCUMENT EXAMINATION94
Questioned document examination involves the comparison and analy-
sis of documents and printing and writing instruments in order to identify
or eliminate persons as the source of the handwriting; to reveal alterations,
additions, or deletions; or to identify or eliminate the source of typewriting
or other impression marks. Questions about documents arise in business,
finance, and civil and criminal trials, and in any matter affected by the in-
tegrity of written communications and records. Typical analyses include:
determining whether the document is the output of mechanical or
electronic imaging devices such as printers, copying machines, and
facsimile equipment;
identifying or eliminating particular human or machine sources of
handwriting, printing, or typewriting;
identifying or eliminating ink, paper, and writing instrument;
establishing the source, history, sequence of preparation, altera-
tions or additions to documents, and relationships of documents;
93 Some relevant questions to be addressed are identified in Bresee, op. cit.
94 This discussion is primarily based on Standard Descriptions of Scope of Work Relating to
Forensic Document Examiners (American Society for Testing and Materials [ASTM] Designa-
tion E 444-98) (1998), Standard Guide for Test Methods for Forensic Writing Ink Comparison
(ASTM Designation E 1422-01) (2001), Standard Guide for Writing Ink Identification (ASTM
Designation E 1789-04) (2004), and Standard Guide for Examination of Handwritten Items
(ASTM Designation E 2290-03) (2003).
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STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
deciphering and restoring obscured, deleted, or damaged parts of
documents;
recognizing and preserving other physical evidence that may be
present in documents; and
determining the age of a document.
Questioned document examiners are also referred to as forensic docu-
ment examiners or handwriting experts; questioned document examination
includes the field of handwriting identification, while handwriting includes
cursive or script style writing, printing by hand, signatures, numerals, or
other written marks or signs. Forensic document examination does not
involve a study of handwriting that purports to create a personality profile
or otherwise analyze or judge the writer’s personality or character.
Analyses
Equipment used in questioned document examination includes micro-
scopes and other optical aids, photographic and other imaging devices,
and a wide variety of imaging materials adaptable for use with numerous
lighting methods, including those involving ultraviolet, visible, and infrared
light, and other regions of the electromagnetic spectrum. Software tools re-
cently have become available for the analysis of handwriting.95 The analysis
of papers and inks is similar to other forensic chemistry work. The principal
procedures used for ink examination are nondestructive optical examina-
tions and chemical examinations. Optical examinations include those that
use visible and alternative light sources—for example, determining whether
the class of ink is ballpoint pen; using ultraviolet examination to reveal
indications that a document has been stained by chemicals; and employing
reflected infrared to observe luminescence at different wavelengths. Chemi-
cal examination includes spot testing during which solvents are applied in
small amounts to the ink line. For example, ballpoint inks, which are either
oil based or glycol based, are highly soluble in pyridine. Inks formulated
for fountain pens, porous point pens, and roller pens generally are water
soluble in ethanol and water. Indelible markers are solvent based and gener-
ally would be soluble in pyridine.
Ink examination can have one of two objectives: class identification—
for which the intention is to identify the ink formula or type based on a
reference library of samples of inks—and comparison, for which the goal
is to compare two ink samples to determine whether they are of common
95 For an overview, see S.N. Srihari and G. Leedham. 2003. A survey of computer methods in
forensic document examination. Proceedings of the 11th International Graphonomics Society
Conference, pp. 278-281. Available at www.ntu.edu.sg/sce/labs/forse/PDF/docExam_7.pdf.
FORENSIC SCIENCE DISCIPLINES
165
origin. Ink comparisons usually are performed to answer four basic cat-
egories of questions: (1) whether an ink is the same (in formula) as that
on other parts of the same document or on other documents; (2) whether
two writings with similar ink have a common origin (e.g., the same writing
instrument or ink well); (3) whether the ink of entries over a period of time
is consistent with varying ages or indicates preparation at one time; and (4)
whether ink is as old as it purports to be.
Most problems with ink examinations arise from confounding factors
that interact with the ink. These can be part of the writing process, such as
blotting wet ink; variations in the papers; various forms of contamination
on the document; or a combination of these factors. Most ink examinations
must be performed on paper and without defacing the handwriting, and this
creates a number of sampling and analytical challenges.
The examination of handwritten items typically involves the compari-
son of a questioned item submitted for examination along with a known
item of established origin associated with the matter under investigation.
Requirements for comparison are that the writing be of the same type
(handwritten/cursive versus hand printed) and that it be comparable text
(similar letter/word combinations). Special situations involving unnatural
writing are forgery (an attempt to imitate/duplicate the writing of another
person) and disguise (an attempt to avoid identification as the writer). The
basis for comparison is that handwriting/handprinting/numerals can be
examined to obtain writing characteristics (also referred to as features or
attributes). The characteristics are further classified into class characteristics
(the style that the writer was taught), individual characteristics (the writer’s
personal style), and gross/subtle characteristics.
Specific attributes used for comparison of handwriting are also referred
to as discriminating elements, of which Huber and Headrick have identified
21.96 Comparisons are based on the high likelihood that no two persons
write the same way, while considering the fact that every person’s writing
has its own variabilities. Thus, an analysis of handwriting must compare
interpersonal variability—some characterization of how handwriting fea-
tures vary across a population of possible writers—with intrapersonal
variability—how much an individual’s handwriting can vary from sample
to sample. Determining that two samples were written by the same person
depends on showing that their degree of variability, by some measure,
is more consistent with intrapersonal variability than with interpersonal
variability. Some cases of forgery are characterized by signatures with too
little variability, and are thus inconsistent with the fact that we all have
intrapersonal variability in our writing.
96 R.A. Huber and A. M. Headrick. 1999. Handwriting Identification: Facts and Fundamen-
tals. Boca Raton, FL: CRC Press.
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STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
Scientific Interpretation and Reporting of Results
Terminology has been developed for expressing the subjective conclu-
sions of handwriting comparison and identification, taking into account
that there are an infinite number of gradations or opinions toward an
identification or elimination. Several scales, such as a five-point scale and a
nine-point scale, are used by questioned document examiners worldwide.
The nine-point scale is as follows:
1. Identification (a definite conclusion that the questioned writing
matches another sample)
2. Strong probability (evidence is persuasive, yet some critical quality
is missing)
3. Probable (points strongly towards identification)
4. Indications [that the same person] did [create both samples] (there
are a few significant features)
5. No conclusion (used when there are limiting factors such as dis-
guise, or lack of comparable writing)
6. Indications [that the same person] did not [create both samples]
(same weight as indications with a weak opinion)
7. Probably did not (evidence is quite strong)
8. Strong probably did not (virtual certainty)
9. Elimination (highest degree of confidence)97
Summary Assessment
The scientific basis for handwriting comparisons needs to be strength-
ened.98 Recent studies have increased our understanding of the individuality
and consistency of handwriting and computer studies99 and suggest that
97 Standard Terminology for Expressing Conclusions of Forensic Document Examiners,
ASTM Designation E 1658-04.
98 M. Kam, G. Fielding, and R. Conn. 1997. Writer identification by professional document
examiners. Journal of Forensic Sciences 42(5):778-786, reports on proficiency tests given to
more than 100 questioned document examiners and to a control group of individuals with
similar educational backgrounds. Each subject made 144 pair-wise comparisons. Although
the study showed that document examiners are much more accurate than lay people in de-
termining whether or not two samples “match” (based on the “identification” and “strong
probability” definitions of ASTM standard E1658), professionals nonetheless declared an
erroneous match in 6.5 percent of the comparisons. A similar, more recent study, focusing on
whether individual signatures were genuine, is reported in J. Sita, B. Found, and D. Rogers.
2002. Forensic handwriting examiners’ expertise for signature comparison. Journal of Foren-
sic Sciences 47:1117. That study found that professional handwriting examiners erred in 3.4
percent of their judgments.
99 E.g., S.N. Sargur, S.-H. Cha, H. Arora, and S. Lee. 2002. Individuality of handwriting.
Journal of Forensic Sciences 47(4):1-17.
FORENSIC SCIENCE DISCIPLINES
167
there may be a scientific basis for handwriting comparison, at least in the
absence of intentional obfuscation or forgery. Although there has been only
limited research to quantify the reliability and replicability of the practices
used by trained document examiners, the committee agrees that there may
be some value in handwriting analysis.
Analysis of inks and paper, being based on well-understood chemistry,
presumably rests on a firmer scientific foundation. However, the committee
did not receive input on these fairly specialized methods and cannot offer a
definitive view regarding the soundness of these methods or of their execu-
tion in practice.
ANALYSIS OF PAINT AND COATINGS EVIDENCE
Paint is a suspension of solid pigments in a polymeric binder that, after
application by brushing, spraying, dipping, or other means, forms a protec-
tive and/or decorative coating. When two objects come in contact with one
another and at least one of these objects is painted, a transfer of paint may
occur. This transferred paint can be compared to the paint located near the
point of damage to determine if the two samples have a common origin.
Painted surfaces tend to be repainted over time, providing a characteristic
history of layer sequence. Painted surfaces are encountered frequently at
crime scenes in the form of vehicles, architectural structures, tools, bicycles,
boats, and many other items. The results of the examinations often are
valuable both during the investigation and as evidence if a trial results.
Paint examinations by their nature can be useful in suggesting possible con-
nections of evidence from the crime scene to its source and therefore are
helpful in narrowing or excluding possible witnesses and suspects as well
as in providing useful information for investigative leads.
Sample Data and Collection
There are many different types of paint and other coatings, including
architectural, vehicular, and marine. Evidence collected from the crime
scene may include painted surfaces such as automotive panels, tools, or
victims’ or suspects’ clothing, or spray paint, smears, chips, or flakes. After
documentation at the scene, the damaged painted surface is protected and
preserved and then submitted to the laboratory. When it is not possible to
bring the painted item or a portion of it to the laboratory, paint samples
may be removed in such a way that the entire layer sequence is captured
intact.
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STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
Analyses
The proper recognition and collection of paint evidence at the scene
precedes the comparison of evidence occurring at the laboratory. The color,
texture, type, layer sequence, and chemical composition of known and
questioned paints are compared, and a conclusion is rendered. Additionally,
in cases for which no suspect vehicle and questioned paint are available, it
may be possible to provide at least an investigative lead based on the color
and metallic/nonmetallic type of paint present. If appropriate, the Royal
Canadian Mounted Police’s PDQ (Paint Data Query) database may be
searched, and vehicular information may be provided regarding the pos-
sible makes, models, and year range of vehicles that used the questioned
paint system.
The examination and comparison of paint evidence requires microscopic
and instrumental techniques and methods. The examination of questioned
and known samples follows an analytical process that identifies and com-
pares the class (or group) characteristics of the evidence.100 Occasionally,
identifying characteristics exist across edges that allow edge or piece fitting.
These characteristics include irregular borders, brush stroke striations, pol-
ish mark striations, or surface abrasion markings. When paint fragments
physically fit back to a sample from a known source, the fragments are
identified as having come from that specific source. Only when physical
fitting is possible can an individualized source determination be made
Examiners involved with the analysis of paint evidence in the labora-
tory typically possess an extensive scientific background, because many
of the methods and analyses rely heavily on chemistry.101 The suggested
minimum education requirement is a bachelor’s degree in a natural102,103
or applied science,104 with many candidates possessing a graduate degree.
Coursework needs to include one year (or equivalent) of general chemistry
with laboratory, organic chemistry with laboratory, analytical/instrumental
analysis, and light microscopy to include basic polarized light microscopy—
the latter obtained through structured coursework if it is not available at
the graduate or undergraduate level.105 On-the-job training continues in the
laboratory, with its length depending on the examiner’s experience. Before
examiner trainees can work cases independently, they must observe and
100 SWGMAT. 1999. Forensic paint analysis and comparison guidelines. Forensic Science
Communications 1(2). Available at www.fbi.gov/hq/lab/fsc/backissu/july1999/painta.htm.
101 SWGMAT. 2000. Trace evidence quality assurance guidelines. Forensic Science Com-
102 G.S. Anderson (ed.). Canadian Society of Forensic Science. 2007. CSFS Careers in Foren-
103 SWGMAT 2000, op. cit.
104 Ibid.
105 Ibid.
FORENSIC SCIENCE DISCIPLINES
169
work under the supervision of an experienced examiner. The completion
of a laboratory’s training program in paint analysis can range between 12
to 18 months.106
Scientific Interpretation and Reporting of Results
SWGMAT sets guidelines for this field, but it has not recommended
report wording, and there are no set criteria for determining a conclusion,
although a range of conclusions may be used to show the significance of the
examination results. The strength of a conclusion depends on such variables
as the number of layers present, the sample condition, and the type of paint
(vehicular or structural). Terms such as “matched,” “indistinguishable,”
“consistent,” or “similar” are used along with the properties of the paints
that were compared in stating the results of the comparison.
If there are no significant differences in the properties compared, the
examiners may conclude that the paint or coating samples could have had
a common origin. This does not mean they came from the same source to
the exclusion of all others, but rather that they may have originated from
the same source or from different sources that were painted or coated in the
same manner. As the number of different layers associated increases (e.g.,
multiple different layers on a repainted surface), it may be concluded that it
is unlikely that the questioned paint originated from any source other than
that of the known paint.
SWGMAT has suggested forensic paint analysis and comparison guide-
lines107,108 that discuss the examination procedure and instrumentation op-
tions, and ASTM has published the general guidelines.109 However, neither
includes report wording suggestions. Additional work should be done to
provide standard language for reporting conclusions and sources of uncer-
tainty. Such work has been completed by working groups for other forensic
disciplines. Proficiency testing requirements are agreed upon by the predom-
inant accrediting organization, the American Society of Crime Laboratory
Directors-Laboratory Accreditation Board (ASCLD/LAB), which requires
testing (internal or external) once per calendar year.
106 Anderson, op. cit.; SWGMAT.
107 SWGMAT. 1999. Forensic paint analysis and comparison guidelines. Forensic Science
Communications 1(2). Available at www.fbi.gov/hq/lab/fsc/backissu/july1999/painta.htm.
108 SWGMAT. 2002. Standard guide for using scanning electron microscopy/X-ray spec-
trometry in forensic paint examinations. Forensic Science Communications 4(4). Available at
109 Ibid.
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STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
Summary Assessment
As is the case with fiber evidence, analysis of paints and coatings is
based on a solid foundation of chemistry to enable class identification. Vi-
sual and microscopic examinations are typically the first step in a forensic
examination of paints and coatings because of the ability to discriminate
paints/coatings based on properties determined with these examinations.
Several studies have been conducted that included hundreds of random
automotive paint samples.110 These studies have concluded that more than
97 percent of the samples could be differentiated based on microscopic
examinations coupled with solubility and microchemical testing. Another
study111 determined that more than 99 percent of 2,000 architectural paint
samples could be similarly differentiated. However, the community has not
defined precise criteria for determining whether two samples come from a
common source class.
ANALYSIS OF EXPLOSIVES EVIDENCE AND FIRE DEBRIS
Explosives evidence encompasses a wide range of materials from un-
burned, unconsumed powders, liquids, and slurries, to fragments of an ex-
plosive device, to objects in the immediate vicinity of an explosion thought
to contain residue from the explosive. A typical analytical approach would
be to identify the components and construction of an explosive device and
conduct an analysis of any unconsumed explosives and residues. In addition
to the analysis and identification of low and high explosives, chemical reac-
tion bottle bombs are also analyzed. The scene of an explosion can require
special investigative attention. What may appear to be a small piece of scrap
metal could in fact be an important piece of the device that caused the ex-
plosion. The very nature of an explosion has a direct impact on the quality
of evidence recovered. Pristine devices or device fragments, or appreciable
amounts of unconsumed explosive material, should not be expected.
Analyses
Generally speaking, laboratories will not accept devices until they have
been rendered safe. Examiners involved with the analysis of explosives evi-
dence in the laboratory typically have an extensive scientific background,
because the methods used entail a large amount of chemistry and instru-
110 S.G. Ryland and R.J. Kopec. 1979. The evidential value of automobile paint chips. Jour-
nal of Forensic Sciences 24(1):140-147; J.A. Gothard. 1976. Evaluation of automobile paint
flakes as evidence. Journal of Forensic Sciences 21(3):636-641.
111 C.F. Tippet. 1968. The evidential value of the comparison of paint flakes from sources
other than vehicles. Journal of the Forensic Sciences Society 8(2-3):61-65.
FORENSIC SCIENCE DISCIPLINES
171
mentation. The Technical Working Group for Fire and Explosives (TW-
GFEX), a group of fire debris and explosives examiners, suggests that an
explosives examiner be required to possess a bachelor’s degree in a natural
or applied science, with recommended coursework in chemistry and instru-
mental analysis.112 The group also recommends that the examiner complete
a training program that includes the analysis of low and high explosives,
instruction in the use of instrumentation used in routine analyses, the con-
struction of explosive devices, and participation in a postblast investigation
course. Although there is no official certification program for explosives
examiners, TWGFEX has devised a suggested training guide. The guide is
divided into seven modules, each with a reading list, practical exercises,
and methods of evaluation.113 To ensure that examiners maintain a level
of competency, proficiency testing (internal or external) is required by AS-
CLD/LAB once per calendar year.114
The ultimate goal of an explosives examination is the identification of
the explosive material used, whether it is through the analysis of an intact
material or of the residue left behind when the material explodes. Intact
material lends itself to being more easily identified. The individual compo-
nents of postblast residue may often be identified (e.g., potassium chloride
and potassium sulfate). The training and experience of examiners allows
them to deduce what types of explosive material were originally present
from possible combinations of explosive materials.
Whether it is a low explosive or high explosive, the analysis of an intact
explosive material follows a procedure that begins with a macroscopic and
microscopic examination of the material, followed by a burn test, when ap-
propriate. The results of the initial observations will dictate how the rest of
the analysis will proceed. Typically it will involve the use of instrumentation
that provides both elemental and structural information about the material,
such as X-ray diffraction, scanning electron microscope-energy dispersive
X-ray analysis, or infrared spectroscopy. TWGFEX has devised guidelines
for the analysis of intact explosives that categorize the instruments that can
be used based on the level of information they provide.115 The information
gathered, if sufficient, can be useful in identifying the material.
The analysis of postblast explosive residues begins much like the analy-
112 TWGFEX Explosive Examiners Job Description. Undated. Available at http://ncfs.ucf.
edu/twgfex/documents.html.
113 TWGFEX Training Guide for Explosives Analysis Training. Undated. Available at http://
ncfs.ucf.edu/twgfex/Documents.html.
114 American Society of Crime Laboratory Directors International. 2006. Supplemental
Requirements for the Accreditation of Forensic Science Testing Laboratories, p. 20. See www.
ascld-lab.org/international/indexinternational.html.
115 TWGFEX Recommended Guidelines for Forensic Identification of Intact Explosives.
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STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
sis of intact explosives, with the macroscopic and microscopic analysis of
the evidence submitted (whether it is an expended device, fragments of a
device, or debris from near the site of the explosion). If no intact explosive
material is found, a sequence of extracts may be used to capture any organic
and/or inorganic residues present. These extracts are then analyzed em-
ploying the same instrumentation used for intact explosives. However, the
results produced differ in their specificity, and it is here that the training and
expertise of the examiner plays a large role. To interpret the results prop-
erly, the examiner must have knowledge of the composition of explosives
and the reaction products that form when they explode. Interpretation can
be further complicated by the presence of contaminants from, for example,
the device or soil.116
Examination conclusions for postblast residues range from “the resi-
due present was consistent with an explosive material” to “the residue is
only indicative of an explosive” to “no explosive residues were present.”
TWGFEX recently has developed a set of guidelines for the analysis of
postblast explosive residues,117 but has yet to make any recommendations
for report wording.
The examination of fire debris not associated with explosions often
aims to determine whether an accelerant was used. To assess the effects of
an accelerant, one might design an experiment, under a range of conditions
(e.g., wind speed, temperature, presence/absence of other chemicals) with
two groups: one in which materials are burned in the presence of an accel-
erant (“treatment”) and one with no accelerant (“control”). The measured
outcomes on the burned materials might be measures that characterize the
damage patterns (e.g., depth of char, size of bubbles on surfaces). Differ-
ences in the ranges of these measurements from the materials in the two
groups (treatment versus control) suggest a hypothesis about the effects
of an accelerant. Following this exploration, one should design validation
studies to confirm that these measures do indeed characterize the differences
in materials treated or untreated with an accelerant.
Summary Assessment
The scientific foundations exist to support the analysis of explosions,
because such analysis is based primarily on well-established chemistry. As
part of the laboratory work, an analyst often will try to reconstruct the
bomb, which introduces procedural complications, but not scientific ones.
116 C.R. Midkiff. 2002. Arson and explosive investigation. In: R. Saferstein (ed.). Forensic
Science Handbook. Vol. 1, 2nd ed. Upper Saddle River, NJ: Prentice Hall.
117 TWGFEX Recommended Guidelines for Forensic Identification of Post-Blast Explosive
Residues. 2007. Available at http://ncfs.ucf.edu/twgfex/action_items.html.
FORENSIC SCIENCE DISCIPLINES
173
By contrast, much more research is needed on the natural variability
of burn patterns and damage characteristics and how they are affected by
the presence of various accelerants. Despite the paucity of research, some
arson investigators continue to make determinations about whether or not
a particular fire was set. However, according to testimony presented to the
committee,118 many of the rules of thumb that are typically assumed to
indicate that an accelerant was used (e.g., “alligatoring” of wood, specific
char patterns) have been shown not to be true.119 Experiments should be
designed to put arson investigations on a more solid scientific footing.
FORENSIC ODONTOLOGY
Forensic odontology, the application of the science of dentistry to the
field of law, includes several distinct areas of focus: the identification of
unknown remains, bite mark comparison, the interpretation of oral injury,
and dental malpractice. Bite mark comparison is often used in criminal
prosecutions and is the most controversial of the four areas just mentioned.
Although the identification of human remains by their dental characteristics
is well established in the forensic science disciplines, there is continuing
dispute over the value and scientific validity of comparing and identifying
bite marks.120
Many forensic odontologists providing criminal testimony concerning
bite marks belong to the American Board of Forensic Odontology (ABFO),
which was organized in 1976 and is recognized by the American Academy
of Forensic Sciences as a forensic specialty. The ABFO offers board certifi-
cation to its members.121
Sample Data and Collection
Bite marks are seen most often in cases of homicide, sexual assault,
and child abuse. The ABFO has approved guidelines for the collection of
evidence from bite mark victims and suspected biters.122 The techniques
for obtaining bite mark evidence from human skin—for example, various
forms of photography, dental casts, clear overlays, computer enhancement,
electron microscopy, and swabbing for serology or DNA—generally are
118 J. Lentini. Scientific Fire Analysis, LLC. Presentation to the committee. April 23, 2007.
Available at www7.nationalacademies.org/stl/April%20Forensic%20Lentini.pdf.
119 NFPA 921 Guide for Explosion and Fire Investigations, 2008 Edition. Quincy, MA:
National Fire Protection Association.
120 E.g., J.A. Kieser. 2005. Weighing bitemark evidence: A postmodern perspective. Journal
of Forensic Science, Medicine, and Pathology 1(2):75-80.
121 American Board of Forensic Odontology at www.abfo.org.
122 Ibid.
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STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
well established and relatively noncontroversial. Unfortunately, bite marks
on the skin will change over time and can be distorted by the elasticity of
the skin, the unevenness of the surface bite, and swelling and healing. These
features may severely limit the validity of forensic odontology. Also, some
practical difficulties, such as distortions in photographs and changes over
time in the dentition of suspects, may limit the accuracy of the results.123
Analyses
The guidelines of the ABFO for the analysis of bite marks list a large
number of methods for analysis, including transillumination of tissue,
computer enhancement and/or digitalization of the bite mark or teeth, ste-
reomicroscopy, scanning electron microscopy, video superimposition, and
histology.124 The guidelines, however, do not indicate the criteria necessary
for using each method to determine whether the bite mark can be related
to a person’s dentition and with what degree of probability. There is no
science on the reproducibility of the different methods of analysis that lead
to conclusions about the probability of a match. This includes reproduc-
ibility between experts and with the same expert over time. Even when
using the guidelines, different experts provide widely differing results and
a high percentage of false positive matches of bite marks using controlled
comparison studies.125
No thorough study has been conducted of large populations to estab-
lish the uniqueness of bite marks; theoretical studies promoting the unique-
ness theory include more teeth than are seen in most bite marks submitted
for comparison. There is no central repository of bite marks and patterns.
Most comparisons are made between the bite mark and dental casts of an
individual or individuals of interest. Rarely are comparisons made between
the bite mark and a number of models from other individuals in addition to
those of the individual in question. If a bite mark is compared to a dental
cast using the guidelines of the ABFO, and the suspect providing the dental
cast cannot be eliminated as a person who could have made the bite, there
is no established science indicating what percentage of the population or
subgroup of the population could also have produced the bite. This follows
from the basic problems inherent in bite mark analysis and interpretation.
As with other “experience-based” forensic methods, forensic odontol-
ogy suffers from the potential for large bias among bite mark experts in
evaluating a specific bite mark in cases in which police agencies provide
the suspects for comparison and a limited number of models from which
123 Rothwell, op. cit.
124 American Board of Forensic Odontology, op. cit.
125 Bowers, op. cit.
FORENSIC SCIENCE DISCIPLINES
175
to choose from in comparing the evidence. Bite marks often are associated
with highly sensationalized and prejudicial cases, and there can be a great
deal of pressure on the examining expert to match a bite mark to a suspect.
Blind comparisons and the use of a second expert are not widely used.
Scientific Interpretation and Reporting of Results
The ABFO has issued guidelines for reporting bite mark comparisons,
including the use of terminology for conclusion levels, but there is no in-
centive or requirement that these guidelines be used in the criminal justice
system. Testimony of experts generally is based on their experience and
their particular method of analysis of the bite mark. Some convictions based
mainly on testimony by experts indicating the identification of an individual
based on a bite mark have been overturned as a result of the provision of
compelling evidence to the contrary (usually DNA evidence).126
More research is needed to confirm the fundamental basis for the sci-
ence of bite mark comparison. Although forensic odontologists understand
the anatomy of teeth and the mechanics of biting and can retrieve sufficient
information from bite marks on skin to assist in criminal investigations and
provide testimony at criminal trials, the scientific basis is insufficient to
conclude that bite mark comparisons can result in a conclusive match. In
fact, one of the standards of the ABFO for bite mark terminology is that,
“Terms assuring unconditional identification of a perpetrator, or without
doubt, are not sanctioned as a final conclusion.”127
Some of the basic problems inherent in bite mark analysis and inter-
pretation are as follows:
(1) The uniqueness of the human dentition has not been scientifically
established.128
(2) The ability of the dentition, if unique, to transfer a unique pattern
to human skin and the ability of the skin to maintain that unique-
ness has not been scientifically established.129
i.
The ability to analyze and interpret the scope or extent of
distortion of bite mark patterns on human skin has not been
demonstrated.
ii. The effect of distortion on different comparison techniques is
not fully understood and therefore has not been quantified.
126 Bowers, op. cit.
127 American Board of Forensic Odontology, op. cit.
128 Senn, op. cit.
129 Ibid.
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STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
(3) A standard for the type, quality, and number of individual charac-
teristics required to indicate that a bite mark has reached a thresh-
old of evidentiary value has not been established.
Summary Assessment
Despite the inherent weaknesses involved in bite mark comparison, it
is reasonable to assume that the process can sometimes reliably exclude
suspects. Although the methods of collection of bite mark evidence are
relatively noncontroversial, there is considerable dispute about the value
and reliability of the collected data for interpretation. Some of the key ar-
eas of dispute include the accuracy of human skin as a reliable registration
material for bite marks, the uniqueness of human dentition, the techniques
used for analysis, and the role of examiner bias.130 The ABFO has devel-
oped guidelines for the analysis of bite marks in an effort to standardize
analysis,131 but there is still no general agreement among practicing forensic
odontologists about national or international standards for comparison.
Although the majority of forensic odontologists are satisfied that bite
marks can demonstrate sufficient detail for positive identification,132 no
scientific studies support this assessment, and no large population studies
have been conducted. In numerous instances, experts diverge widely in their
evaluations of the same bite mark evidence,133 which has led to questioning
of the value and scientific objectivity of such evidence.
Bite mark testimony has been criticized basically on the same grounds
as testimony by questioned document examiners and microscopic hair ex-
aminers. The committee received no evidence of an existing scientific basis
for identifying an individual to the exclusion of all others. That same find-
ing was reported in a 2001 review, which “revealed a lack of valid evidence
to support many of the assumptions made by forensic dentists during bite
mark comparisons.”134 Some research is warranted in order to identify
the circumstances within which the methods of forensic odontology can
provide probative value.
130 Ibid.
131 American Board of Forensic Odontology, op. cit.
132 I.A. Pretty. 2003. A Web-based survey of odontologists’ opinions concerning bite mark
analyses. Journal of Forensic Sciences 48(5):1-4.
133 C.M. Bowers. 2006. Problem-based analysis of bite mark misidentifications: The role of
DNA. Forensic Science International 159 Supplement 1:s104-s109.
134 I.A. Pretty and D. Sweet. 2001. The scientific basis for human bitemark analyses—A
critical review. Science and Justice 41(2):85-92. Quotation taken from the abstract.
FORENSIC SCIENCE DISCIPLINES
177
BLOODSTAIN PATTERN ANALYSIS
Understanding how a particular bloodstain pattern occurred can be
critical physical evidence, because it may help investigators understand
the events of the crime. Bloodstain patterns occur in a multitude of crime
types—homicide, sexual battery, burglary, hit-and-run accidents—and are
commonly present. Bloodstain pattern analysis is employed in crime recon-
struction or event reconstruction when a part of the crime scene requires
interpretation of these patterns.
However, many sources of variability arise with the production of
bloodstain patterns, and their interpretation is not nearly as straightfor-
ward as the process implies. Interpreting and integrating bloodstain pat-
terns into a reconstruction requires, at a minimum:
an appropriate scientific education;
knowledge of the terminology employed (e.g., angle of impact,
arterial spurting, back spatter, castoff pattern);
an understanding of the limitations of the measurement tools used
to make bloodstain pattern measurements (e.g., calculators, soft-
ware, lasers, protractors);
an understanding of applied mathematics and the use of significant
figures;
an understanding of the physics of fluid transfer;
an understanding of pathology of wounds; and
an understanding of the general patterns blood makes after leaving
the human body.
Sample Data and Collection
Dried blood may be found at crime scenes, deposited either through
pooling or via airborne transfer (spatter). The patterns left by blood can
suggest the kind of injury that was sustained, the final movements of a
victim, the angle of a shooting, and more. Bloodstains on artifacts such as
clothing and weapons may be crucial to understanding how the blood was
deposited, which can indicate the source of the blood. For example, a stain
on a garment, such as a shirt, might indicate contact between the person
who wore the shirt and a bloody object, while tiny droplets of blood might
suggest proximity to a violent event, such as a beating.
Analyses
Bloodstain patterns found at scenes can be complex, because although
overlapping patterns may appear simple, in many cases their interpreta-
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STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
tions are difficult or impossible. 135,136 Workshops teach the fundamentals
of basic pattern formation and are not a substitute for experience and ex-
perimentation when applying knowledge to crime reconstruction.137 Such
workshops are more aptly applicable for the investigator who needs to
recognize the importance of these patterns so that he or she may enlist the
services of a qualified expert. These courses also are helpful for attorneys
who encounter these patterns in the course of preparing a case or when
preparing to present testimony in court.
Although there is a professional society of bloodstain pattern ana-
lysts, the two organizations that have or recommend qualifications are
the IAI and the Scientific Working Group on Bloodstain Pattern Analysis
(SWGSTAIN). SWGSTAIN’s suggested requirements for practicing blood-
stain pattern analysis are outwardly impressive, as are IAI’s 240 hours of
course instruction. But the IAI has no educational requirements for certifi-
cation in bloodstain pattern analysis.138 This emphasis on experience over
scientific foundations seems misguided, given the importance of rigorous
and objective hypothesis testing and the complex nature of fluid dynamics.
In general, the opinions of bloodstain pattern analysts are more subjective
than scientific. In addition, many bloodstain pattern analysis cases are
prosecution driven or defense driven, with targeted requests that can lead
to context bias.
Summary Assessment
Scientific studies support some aspects of bloodstain pattern analysis.
One can tell, for example, if the blood spattered quickly or slowly, but some
experts extrapolate far beyond what can be supported. Although the trajec-
tories of bullets are linear, the damage that they cause in soft tissue and the
complex patterns that fluids make when exiting wounds are highly variable.
For such situations, many experiments must be conducted to determine
what characteristics of a bloodstain pattern are caused by particular actions
during a crime and to inform the interpretation of those causal links and
135 H.L. MacDonell. 1997. Bloodstain Patterns. Corning, NY: Laboratory of Forensic
Science; S. James. 1998. Scientific and Legal Applications of Bloodstain Pattern Interpreta-
tion. Boca Raton, FL: CRC Press; P. Pizzola, S. Roth, and P. DeForest. 1986. Blood drop
dynamics-II. Journal of Forensic Sciences 31(1): 36-49.
136 Ibid.; R.M. Gardner. 2004. Practical Crime Scene Processing and Investigation. Boca
Raton, FL: CRC Press; H.C. Lee; T. Palmbach and M.T. Miller. 2005. Henry Lee’s Crime Scene
Handbook. Burlington, MA: Elsevier Academic Press, pp. 281-298.
137 W.J. Chisum and B.E. Turvey. 2007. Crime Reconstruction. Burlington, MA: Elsevier
Academic Press.
138 See “Bloodstain Pattern Examiner Certification Requirements.” Available at theiai.org/
certifications/bloodstain/requirements.php.
FORENSIC SCIENCE DISCIPLINES
179
their variabilities. For these same reasons, extra care must be given to the
way in which the analyses are presented in court. The uncertainties associ-
ated with bloodstain pattern analysis are enormous.
AN EMERGING FORENSIC SCIENCE DISCIPLINE:
DIGITAL AND MULTIMEDIA ANALYSIS
The analysis of digital evidence deals with gathering, processing, and
interpreting digital evidence, such as electronic documents, lists of phone
numbers and call logs, records of a device’s location at a given time, e-
mails, photographs, and more. In addition to traditional desktop and lap-
top computers, digital devices that store data of possible value in criminal
investigations include cell phones, GPS devices, digital cameras, personal
digital assistants (PDAs), large servers and storage devices (e.g., RAIDS and
SANS), video game consoles (e.g., PlayStation and Xbox), and portable
media players (e.g., iPods). The storage media associated with these devices
currently fall into three broad categories. The first, magnetic memory, in-
cludes hard drives, floppy discs, and tapes. The second, optical memory,
includes compact discs (CDs), and digital versatile discs (DVDs). The third,
electrical storage, includes USB flash drives, some memory cards, and some
microchips. These items are the most commonly encountered in criminal
and counterintelligence matters, but laboratories have been asked to ex-
amine such items as scuba dive watches in death investigations and black
boxes in aircraft mishaps.
The proliferation of computers and related devices over the past 30
years has led to significant changes in and the expansion of the types of
criminal activities that generate digital evidence. Initially, computers were
either the weapon or the object of the crime. In the early days, most com-
puter crime involved manipulating computer programs of large businesses
in order to steal money or other resources. As computers became more
popular, they became storage containers for evidence. Drug dealers, book
makers, and white collar criminals began to keep computerized spread-
sheets detailing their transactions. Digital cameras and the Internet have
made child pornography increasingly available, and computers act as a
digital file cabinet to hold this contraband material. Finally, digital media
have become witnesses to daily activities. Many individuals have two cell
phones with text messaging and/or e-mail capability, several computers, a
home alarm system, a GPS in the car, and more; even children often possess
some subset of these items. Workplaces use magnetic card readers to permit
access to buildings. Most communication involves some kind of computer,
and by the end of each day, hundreds of megabytes of data may have been
generated about where individuals have been, how fast they got there, to
whom they spoke, and even what was said. Suicide notes are written on
180
STRENGTHENING FORENSIC SCIENCE IN THE UNITED STATES
computers. Sexual predators stalk their victims online via e-mail, chat, and
instant messaging. Even get-away cars are equipped with GPS devices. Fi-
nally, computer systems have become (with ever-increasing frequency) the
victims of unauthorized control or intrusions. These intrusions often result
in the manipulation of files and the exfiltration of sensitive information. In
addition, computers in automobiles that track speed, breaking, and turn-
ing are valuable in accident reconstruction. As a result, almost every crime
could have digital evidence associated with it.
Sample Data and Collection
The best practices for the collection of digital evidence most often
call for the person at the scene to disconnect the power cord for the com-
puter and related peripheral equipments (e.g., monitor, printer) and seize
these items, as well as any loose storage media such as thumb drives and
CDs. This method works well in most cases. However, some data (like
recently typed passwords, malicious programs, and active communication
programs) are volatile and are stored in the electronic chips of the system.
In these circumstances, this information is lost when the device is turned
off. In intrusion investigations or in cases in which encryption software is
being used, this volatile information could be the key to a successful analy-
sis and prosecution.139
Recognizing potential sources of digital evidence is also an ongoing
challenge. Investigators are likely to seize a desktop computer but walk past
a PlayStation. Thumb drives can be fashioned to look like a pocket knife,
writing pen, or even a piece of sushi. Cell phones and wireless Internet
capability present another challenge: If these devices are turned on while
in law enforcement custody, they could be remotely accessed and altered
by a suspect.
Analyses
The typical approach to examining a computer involves two main
phases. The first is the imaging phase. During this process, the storage
device (most often a hard drive) is fitted with an appliance that prevents
any new information from being written. Then, all of the data are copied
to a new blank hard drive. The copy is compared with the original, most
often by using a mathematical algorithm called Message Digest-5, other-
wise known as MD5 Hash. The MD5 Hash value gives a unique series of
numbers and letters for every file. In the examination phase, this forensi-
139 See W.G. Kruse and J.G. Heiser. 2001. Computer Forensics: Incident Response Essen-
tials. Boston: Addison-Wesley.

 

 

 

 

 

 

 

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